Journal: Scientific Reports

Article Title: Tuning IL-2 signaling by ADP-ribosylation of CD25

doi: 10.1038/srep08959

Figure Lengend Snippet: Splenocytes from DEREG mice were incubated in the absence or presence of the indicated concentrations of NAD + before staining with fluorochrome-conjugated mAbs directed against CD4 and CD25 (mAb PC61 or mAb 7D4). Gating was performed on CD4 + cells. (a) Representative dot plots of cells incubated in the absence or presence of 12 μM NAD + . (b) Percentages of CD4 + GFP + cells staining with anti-CD25 mAbs plotted as a function of the concentration of added NAD + . Results are representative of two independent experiments.

Article Snippet: Alternatively, after erythrocyte lysis with Ack lysis buffer (Bio Whittaker) and depletion of B-cells with Dynabead-conjugated sheep anti-mouse IgG (Invitrogen), Tregs were positively selected by magnetic cell sorting using PE conjugated anti-CD25 (7D4) and magnetic bead-conjugated anti-PE antibodies according to the manufacturer's instructions (Miltenyi Biotec).

Techniques: Incubation, Staining, Concentration Assay

Journal: Cerebral Cortex (New York, NY)

Article Title: Cadmium versus Lanthanum Effects on Spontaneous Electrical Activity and Expression of Connexin Isoforms Cx26, Cx36, and Cx45 in the Human Fetal Cortex

doi: 10.1093/cercor/bhz163

Figure Lengend Snippet: Expression profile of CX45, CX36, and CX26 transcripts in the human fetal cortex, from 17 to 23 GW. (A) Log10 FPKM values derived by the HISAT-StringTie pathway of data analysis. (B) Log10 normalized counts derived by the STAR-HTSeq-DESeq2 data analysis pathway. (C) Expression profile of 2 predominant transcripts of Cx45 (ENST00000592524.5 [CX45_524.5] and ENST00000426548.5 [Cx45_548.5]) in comparison with Cx36 and Cx26 with HISAT-StringTie pathway of analysis from 17 to 23 GW in the human fetal cortex. In panels A–C, n = 4, 17 GW; n = 2, 18 GW; n = 5, 19 GW; and n = 1, 23 GW. (D) Log10 normalized counts of the Cx and pannexin isoforms expressed in the human fetal cortex, age range of 17–23 GW (STAR-HTSeq-DESeq2 data analysis pathway) (n = 7). (E) Differential expression analysis of human fetal samples at 17 and 19 GW, by the STAR-HTSeq-DESeq2 method (n = 3, 17 GW; n = 2, 19 GW). (F) Sex determination based on XIST expression and ratio of X-to-Y coverage in each human subject used in the study (n = 7). (G) Differential expression analysis of male (n = 4) and female (n = 3) human fetal samples from 17 to 19 GW, by the STAR-HTSeq-DESeq2 method. (H) Differential expression analysis of cortex (n = 3) and full brain (n = 3) human fetal samples at 17 GW by the STAR-HTSeq-DESeq2 method. *Padj < 0.05.

Article Snippet: Primary antibodies were purchased from Alomone Labs, Thermo Fisher Scientific, and Millipore (company, antigen [catalog number]: Alomone Labs, Cx26 [ACC-212], Cx36 [ACC-209], and Cx45 [ACC-207]; Thermo Fisher, Cx26 [512800], Cx36 [516200], Nurr1 [MA1-195], beta-3-tubulin [MA1-19582], and NeuN [MA5-33103]; Millipore, NeuN [MAB377]).

Techniques: Expressing, Derivative Assay, Comparison, Quantitative Proteomics

Journal: International Journal of Molecular Sciences

Article Title: Fucoxanthin Ameliorates Atopic Dermatitis Symptoms by Regulating Keratinocytes and Regulatory Innate Lymphoid Cells

doi: 10.3390/ijms21062180

Figure Lengend Snippet: The characterization of Il-2 producers regulated by FX and TAC. FX stimulated Il-2 production in CD45R + CD25 + ILCregs. Some Il-2 + CD45R + CD25 + ILCregs were observed in TAC treated skin and others were CD45R - CD25 - cells. The panel is focused on the dermis.

Article Snippet: Sections were subjected to immunohistochemistry using antibodies of Alexa Fluor 488 ® -anti-mouse Il-10 (#505013), Brilliant Violet 421TM-anti-mouse TGF-β1 (#141407), Pacific BlueTM-anti-mouse IL-2 (#503820), Alexa Fluor ® 488-anti-GATA3 (#653808), PE-anti-mouse Ly-6A/E (Sca-1) (#108108), PE-anti-mouse IL-33Rα (ST2) (#146608) and PE-anti-mouse/human IL-5 (#504304) (BioLegend, San Diego, CA, USA), FITC-anti-mouse/human CD45R (#11-0452-82) (Thermo Fisher Scientific, Waltham, MA, USA), PE-anti-mouse CD25 (#130-120-766) (Miltenyi Biotech, Bergisch Gladbach, Germany).

Techniques: